Invitro antioxidant and free radical scavenging activity. Free radical scavenging activity, total phenolic content. In vitro nitric oxide scavenging activity of methanol extracts of three bangladeshi medicinal plants rozina parul1, sukalayan kumar kundu 2 and pijush saha2 1. Comparative study of abts radical scavenging activity and. After 20 min incubation at room temperature, read the absorbance at 517 nm. Dpph free radical scavenging activity of scopoletin also increased with increasing concentrations r2. Dpph has two major applications, both in laboratory research. Radical scavenging and antioxidant activity of tannic acid. Free radical scavenging in vitro and biological activity. Dpph 1,1diphenyl2picrylhydrazyl is considered as a stable radical because. The scavenging of reactive oxygen species and the potential for cell protection by functionalized fullerene materials junjie yinb,1, fang laoa,1, peter p.
Total phenol and total flavonoid content in extracts, which reason antioxidant and free radical scavenging activity, was determined spectrophotometrically. In vitro nitric oxide scavenging activity of methanol. What is the best method for radical scavenging assay. Radicalscavenging activity and ferric reducing ability of. Numerous attempts have been made to relate the free radical scavenging capacity of compounds to their antioxidant activity in foods even though antioxidant activity is dependent on both physical and chemical properties. Maisuthisakul p, suttajit m, pongsawatmanit r 2007 assessment of phenolic content and free radical scavenging capacity of some thai indigenous plants. Invitro free radical scavenging activity and bioavailability. The ethanolic extract exhibited higher free radical scavenging effect than the water extract at all tested concentrations. Department of pharmacy, gono bishwabidyalay, savar, dhaka 44, bangladesh.
Dpph free radical scavenging activity dpph is a purple colored stable free radical. Sixteen extracts showed strong antioxidant capabilities, which were, subjected for their dose dependent activity at different concentrations to calculate ic 50 values. Highthroughput relative dpph radical scavenging capacity. Research article comparative invivo free radical scavenging. Evaluation of nitrite radical scavenging properties of. Ftir spectral studies clearly indicate the presence of various functional groups which may be attributed to the antioxidant and free radical scavenging properties of these extracts. Dpph 2, 2diphenyl1 picrylhydrazyl free radical scavenging assay dpph method is also used to.
Antioxidant activity by dpph assay of potential solutions. A number of protocols have been followed for this assay resulting in variation in the results of different laboratories. Free radical scavenging activity of ethanolic extracts. For assessing free radical scavenging potential of p. Triphala ethanolic extract exhibited potent free radical scavenging activity. The experiment has been performed in triplicate, was recorded as mean sd and their variance is analysed using oneway anova procedure as shown in table 1. Wangf, yang qiua, baoyun sunc, gengmei xingc, jinquan dongc, xingjie lianga, chunying chena,c, adivision of nanomedicine and nanobiology, national center for nanoscience and technology. Pdf antioxidant activity by dpph radical scavenging method. The aim of study is to compare the free radical scavenging activity of pineapple extract and eclipta alba extract by no assay. Antioxidant activity and free radicalscavenging capacity. Ethanol extract of paederia foetida was mixed with 95% ethanol to prepare the stock solution 5 mgml. The measurement of the dpph radical scavenging activity was performed according to methodology described by brandwilliams et al. Dpph free radical scavenging activity of the extracts of. Dpph scavenging effect % a 0a 1a 0 x 100 where a 0 was the absorbance of the control reaction and a 1 was the absorbance in the presence of the sample of caffeine, caffeic acid and the combination.
Freshly prepared dpph solution was taken in test tubes and extracts were added followed by serial dilutions 15. Free radical scavenging activities of watersoluble extracts from some natural sources. Radical scavenging and antioxidant activity of ethanolic. Antioxidant activity by dpph assay of potential solutions to. Sanchezmoreno c 2002 methods used to evaluate the free radical scavenging activity in foods and biological systems. The ic 50 values for scavenging of free radicals were 112. The nitrite radical scavenging assay was carried out on the water extracts from a concentration range of 100 to. During the different stages of the brewing process the free radical scavenging activity is changed. Antioxidant and free radical scavenging activity of. Free radical scavenging is associated with the antioxidant potential of a compound. The differences between the free radical scavenging activity of laboratory and production.
The crude extracts were diluted using ethanol according to the assay needs. Hydroxyl radical scavenging activity, nitric oxide radical scavenging activity, reducing power, lipid peroxidation inhibiting activity and total antioxidant assay using standard procedure. Dpph radical scavenging activity the free radical scavenging capacity of the extracts was determined using dpph. The freeradical scavenging activities of these compounds were tested by their ability to bleach the stable radical dpph. The antioxidant activity of the extracts was measured on the basis of the scavenging activity of the stable 1, 1 diphenyl 2picrylhyorazyl dpph free radical according to the method described by brandwilliams et al22 with slight modifications. The scavenging of reactive oxygen species and the potential.
In humans, many diseases are associated with the accumulation of free radicals. The extract showed total antioxidant activity with a trolox equivalent antioxidant concentration teac value of 0. In vitro antioxidant and free radical scavenging activity of. The total phenolic content of the extracts was found to be higher in a. Dpph radicalscavenging methods is common spectrophotometric procedures for. An assessment of the potential of proline to scavenge free radicals was made in a couple of in vitro assay systems, namely graft copolymerization and autooxidation of pyrogallol. Characterization and dpph radical scavenging activity of. It is a darkcolored crystalline powder composed of stable freeradical molecules. A highthroughput relative 2,2diphenyl1picryhydrazyl dpph radical scavenging capacity rdsc assay was developed and validated in the present study.
Free radical scavenging activity screening of medicinal. Antioxidant, dpph, free radical scavenging activity, medicinal plants, northeast india, tripura. The aim of this work is to study and compare the antioxidant properties and phenolic contents of aqueous leaf extracts of juniperus thurifera, juniperus oxycedrus, juniperus phoenicea, and tetraclinis articulata from morocco. Chapter vi evaluation of in vitro free radical scavenging. Dpph radical scavenging activity pph radical is a stable organic free radical with adsorption band at 517 nm. The publisher 2007 tutorial pdf free free radical scavenging activity of antioxidants in foods. Free radical scavenging potential of picrorhiza kurrooa royle. Scavenging of dpph free radical is the basis of a common antioxidant assay. Dpph is a stable free radical that reacts with compounds able to donate a hydrogen atom. Evaluation of antioxidant and free radical scavenging. The samples were reacted with the stable dpph radical in an ethanol solution. The determination of the total antioxidant activity frap assay in. It loses this adsorption when accepting an electron or a free radical species, which results in a visually noticeable discoloration from purple to yellow 9. This rdsc assay is easy to perform and has acceptable accuracy 90.
Antioxidant and free radical scavenging activity of spondias. Both these assays are essentially dependent upon free radical mechanisms. The antioxidant and free radical scavenging activity were determined by several standard methods using spectrophotomer. Dpph free radical scavenging activity of the extracts of the. Free radical scavenging activities of solutions of the plant extracts and synthetic antioxidant substances used in the study prepared in methanol at concentrations of 50, 100 and 200. Free radical scavenging activity of crude extracts and 4. Therefore, the search for naturally occurring antioxidants of plant origin is imperative.
Antioxidant activities of the extracts were evaluated by 2,2diphenyl1picrylhydrazyl dpph free radicalscavenging ability, trolox equivalent. In the present study, the high dpph radical scavenging activity of the. In vitro antioxidant and free radical scavenging activity. Bioactive compounds, radical scavenging, antioxidant. Free radical scavenging in vitro and biological activity of diphenyl diselenideloaded nanocapsules.
Journal of chemical and pharmaceutical research, 2015, 77. Pdf antioxidant activity by dpph radical scavenging. The free radical scavenging activity of the ethanolic extracts was carried out based on a method developed by re et al. Antioxidant and free radical scavenging activity of certain. Antioxidant potential of the plant extract was measured in. The percent dpph scavenging effect was calculated using the following equation. Here, we aimed to investigate the antioxidant and free radical scavenging properties of methanolic extracts from tabebuia pallida t. Materials and methods dpph free radical scavenging activity processing of plants for extract preparation.
Pdf antioxidant and free radical scavenging activities of. Dpph free radical scavenging capacity of legume extracts was evaluated according to the method of chen and ho 11 as modified by xu and chang 10. The free radical scavenging activities of these compounds were tested by their ability to bleach the stable radical dpph. The free radical scavenging activity of methanol extract was measured by 1,1diphenyl2picrylhydrazyl dpph using the method of blois 1958. Lower ec50 value indicates a higher dpph free radical scavenging activity. The antioxidant activity of the memq was evaluated by the phosphomolybdenum method according to the procedure of prieto et al. The observed differences in free radicalscavenging capabilities support the hypothesis that both. There are several assays to measure the antioxidant potential of compounds, the 1,1diphenyl2picrylhydrazine dpph radical scavenging assay being the most extensively used antioxidant assay for plant samples. Free radical scavenging ability of the extracts was tested by dpph radical scavenging assay as described by blois and desmarchelier et al. Free radicalscavenging capacity, antioxidant activity and. Antioxidant and free radical scavenging activities of some fruits article pdf available in journal of complementary and integrative medicine 81 january 2011 with 446 reads how we measure. The objective of this study was to compare the free radical scavenging activity of various compounds to their ability to inhibit lipid oxidation in foods. All the results were compared with same concentration of ascorbic acid as a standard which showed the highest free radical scavenging activity of 18. Rapid assay highthroughput assay scavenging capacity index abstract a new microplateadapted dpph rapid assay was developed to assess the antioxidant capacity of pure compounds and foods.
Free radical scavenging activity of ethanolic extracts from. The assay was carried out in buffered medium methanol. Rapid highthroughput assay to assess scavenging capacity. Synthesis%2c spectroscopic%2c antibacterial and free. The inhibitory percentage of dpph was calculated according to the following equation. The percentage radical scavenging of the nitrite radical by the water extracts is shown in figure 2. Above 100gml, the ethanolic extract showed 80% scavenging activity, similar to control antioxidant compounds quercetin, rutin and lascorbic acid. Invitro antioxidant and free radical scavenging activity of. Antioxidants can scavenge free radicals and minimize their impact. It is a darkcolored crystalline powder composed of stable free radical molecules.
The hydrogen atom donating ability of the plant extractives was determined by the decolorization of methanol solution of 2,2diphenyl1picrylhydrazyl dpph. Free radical scavenging potential of picrorhiza kurrooa. The antioxidant assay scavenging prospects of 2,2diphenyl1picrylhydrazyl dpph radical the antioxidant activity of opdh2 and its synthesized metal complexes were determined using a stable 2,2diphenyl1picrylhydrazyl dpph reagent following a previous. In this assay, a molecule or antioxidant with weak ah bonding will react with a stable free radical dpph 2,2diphenyl1picrylhydrazyl. The extract i mgml showed marked protection up to 66. A modified dpph assay was conducted to evaluate free radical scavenging activity using methanol extract of h. An antioxidant is a molecule that inhibits the oxidation of other molecules.
In this paper, we report about the free radicalscavenging, nitritescavenging and nnitrosamine formation inhibitory activities of ethyl acetate extract eae and nbutanol extract be of c. We present a perspective of the protocols followed by different workers with incongruity in their results and recommend a standard procedure within. The result of dpph free radical scavenging assay suggested that the extracts are capable of scavenging free radicals via electron or hydrogen donating mechanisms and. Hence, the significant decrease in free radical can be attributed to the scavenging ability of methanolic extract of wattakaka volubilis leaves. Relationships between free radical scavenging and antioxidant. Dpph free radical scavenging activity of two extracts from. Selective abts and dpph radical scavenging activity of. The dpph assay measures the ability of a compound to act as. The hopping increases additionally the values of the parameter. The antioxidant and free radicalscavenging activity were measured by 1,1diphenyl2picrylhydrazyl dpph, 2,2azinobis3ethylbenzthiazoline6sulfonic acid abts and phosphomolybdenum methods. Evaluation of antioxidant and free radical scavenging capacities of polyphenolics from pods of caesalpinia pulcherrima.
Total phenolic and flavonoid contents were estimated using folinciocalteu reagent and aluminum chloride colorimetric assay methods, respectively. The water extracts exhibited less free radical scavenging capacity than the ethanol extracts. The result of dpph free radical scavenging assay suggested that the extracts are capable of scavenging free radicals via. Pdf antioxidant and free radical scavenging activities. Dpph radical scavenging capacity of phenolic extracts from. The dpph radical scavenging activity s% was calculated using the following equation. Dpph is a common abbreviation for the organic chemical compound 2,2diphenyl1picrylhydrazyl. The antioxidant activity using the dpph 1, 1diphenyl2picrylhydrazyl assay was assessed by the method of blois8. The antioxidant and free radical scavenging activity of the ethanol extracts of melia dubia hiern.